rabbit polyclonal antibody against ep2  (Cayman Chemical)

Journal: BMC Cancer

Article Title: Comprehensive gene expression analysis of canine invasive urothelial bladder carcinoma by RNA-Seq

doi: 10.1186/s12885-018-4409-3

Figure Lengend Snippet: Detection of EP2 in canine iUC tissues by immunohistochemistry. EP2 expression was observed in the membrane and cytoplasm of primary tumor cells (upper) and peritumoral inflammatory cells (lower). Bar = 50 μm

Article Snippet: A rabbit polyclonal antibody against EP2 (1:100 in dilution, Item No. 101750, Cayman Chemical, Ann Arbor, MI) was used and IHC was performed following previous studies [ , ].

Techniques: Immunohistochemistry, Expressing, Membrane

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: (a) Total lysates from 5×105 BJAB, Akata/EBV−, BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells were immunoblotted for EP1, EP2, EP3, and EP4. Data represents three independent experiments. Tubulin was used as loading control. (b) In parallel experiments, supernatants from the indicated cells were collected to measure the amount of PGE2 secreted by each cell line. (c) The indicated cell lines were stained for EP1, EP2, EP3, and EP4 receptors by immunofluorescence. (d) Mean fluorescent intensity (MFI) of EP1, EP2, EP3, and EP4 receptors in HMVEC-d cells infected with KSHV measured by FACS. Indicated are the MFI for the respective receptor at each time point. The data is representative of three independent experiments.

Article Snippet: Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: Control, Staining, Immunofluorescence, Infection

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: (a) Total lysates from 5×105 BJAB, Akata/EBV−, BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells were immunoblotted for EP1, EP2, EP3, and EP4. Data represents three independent experiments. Tubulin was used as loading control. (b) In parallel experiments, supernatants from the indicated cells were collected to measure the amount of PGE2 secreted by each cell line. (c) The indicated cell lines were stained for EP1, EP2, EP3, and EP4 receptors by immunofluorescence. (d) Mean fluorescent intensity (MFI) of EP1, EP2, EP3, and EP4 receptors in HMVEC-d cells infected with KSHV measured by FACS. Indicated are the MFI for the respective receptor at each time point. The data is representative of three independent experiments.

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: Control, Staining, Immunofluorescence, Infection

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: Effect of concurrent targeting of COX-2 and EP1/EP4 receptors with 1.0µM each of celecoxib, SC-51322, and GW 627368X on various classes of NHL genes

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques:

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: (a) Total lysates from 5×105 BJAB, Akata/EBV−, BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells were immunoblotted for EP1, EP2, EP3, and EP4. Data represents three independent experiments. Tubulin was used as loading control. (b) In parallel experiments, supernatants from the indicated cells were collected to measure the amount of PGE2 secreted by each cell line. (c) The indicated cell lines were stained for EP1, EP2, EP3, and EP4 receptors by immunofluorescence. (d) Mean fluorescent intensity (MFI) of EP1, EP2, EP3, and EP4 receptors in HMVEC-d cells infected with KSHV measured by FACS. Indicated are the MFI for the respective receptor at each time point. The data is representative of three independent experiments.

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: Staining, Immunofluorescence, Infection

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: Effect of concurrent targeting of COX-2 and EP1/EP4 receptors with 1.0µM each of celecoxib, SC-51322. and GW 627368X on various classes of NHL genes.

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: